The scientific activity of Prof. Paolo Borghetti has focused on animal disease both in spontaneous and experimental pathology. Research has consolidated experiences on two main themes:
1) Experimental and applied Immunology immunopathology;
2) In vitro and in vivo studies on condrogenesis and osteogenesis and biocompatibility test on biomaterials used for tissue engineering of cartilage.
The main topic is the study of the immune response in the pig species during viral and mycoplasma infections. Another inherent topic is the evaluation of the immune response during pre- / probiotic treatment. Further characterizing studies are performed on chondrocyte differentiation and to test biomaterials and condrogenic factors for the tissue engineering for cartilage and bone repair.
CABASSI Clotilde Silvia
Research activity is focused on antibiotic resistance, in particular to the detection of multi-resistant bacteria in animals, and on the development and evaluation of new molecules with antimicrobial activity on bacterial reference and field strains and biofilm. This activity is also evidenced by the development and patenting of two new families of antimicrobial peptides(New amino acid sequences with microbicidal activity derived from naja atra cardiotoxin 1 (CTX-1) Cabassi C.S., Sala A., 2016, PCT/EP2016/061022; New cyclic cationic peptides with antimicrobial activity. Baroni M.C., Cabassi C.S., Romani A. 2014. WIPO/PTC - WO 2014/102596 A2).
The research topics of biotechnology field concern:
- the study of in vivo and in vitro experimental models for the evaluation of osteogenesis and osteointegration, cellular and tissue interaction with new generation bioactive surfaces and nanomaterials, cell therapy approaches for osteochondrogenesis;
- histological and anatomical study for the setting up and validation of new animal models in the pre-clinical research lung field.
The applied techniques are morphometric analysis , morphostructural histology and dynamic histomorphometry, SEM and TEM ultrastructural analysis, cytologic assays, cyto- and histo-chemistry, immunofluorescence.
Personal and collaborative research interests of Prof. Cavirani are in understanding the pathogeneses of naturally occurring viral and bacterial diseases of domestic animals, mainly focusing on diagnosis and vaccine based prophylaxis. Active research projects in his lab involve testing hypotheses that define parameters to exploit and compare traditional as well as new generation vaccines. In the broader contest the expertise’s current mission of Prof. Cavirani includes solving problems associated with the application of commercial vaccines using alternative protocols of immunization. Additionally Prof. Cavirani is involved on the discovery and use in silico projected anti-bacterial peptides.
1) Involvement of BoHV-4 in bovine post-partum metritis.
Viruses are an important and common cause of genital tract disease in humans and domestic animals, which affect the quality of life and cause infertility. Bovine herpesvirus 4 (BoHV-4) is a virus that in most species and cell types causes little damage. However BoHV-4 seems to be associated with uterine disease in cattle. We have shown that the virus appears to “hijack” the cellular machinery of uterine stromal and epithelial cells, to drive viral replication, leading to extensive damage. We are mainly focused on elucidate the effect on viral replication by host factors that modulate the uterine environment, and identifythe molecular mechanisms by which the host’s molecules interact with the virus.
2) Development of models for lung inflammation based on in vivo image analysis.
Idiopathic pulmonary fibrosis is a very common interstitial lung disease derived from chronic inflammatory insults, characterized by massive scar tissue deposition that causes the progressive loss of lung function and subsequent death for respiratory failure. We are mainly focused on the generation of an in vivo model based on a reporter gene construct, containing the luciferase open reading frame under different promoter control regions, to be tested on bleomycin-treated mice to investigate, in real time, the correlation between bleomycin treatment, inflammation, tissue remodeling and fibrosis. Bioluminescence emitted by the lungs of bleomycin-treated mice and molecular tomography, successfully allows real time monitoring of fibrosis establishment. The reporter gene technology experienced in our lab in collaboration with a drug company, represent an advanced functional approach for real time non-invasive assessment of disease evolution during therapy, in a reliable and translational living animal model.
animal disease both in spontaneous and experimental pathology:
1) Experimental and applied Immunology, immunopathology;
2) Immunological features in neoplastic diseases
3) Immunophenotype of lymphocytes subpopulations in parasitic diseases of domestic animals
4) In vitro and in vivo studies on condrogenesis and osteogenesis and biocompatibility test on biomaterials used for tissue engineering of cartilage
1) In vitro evaluation of antimicrobial agents;
2) Epidemiological surveys on bacterial diseases of domestic and wild animals.