Viral vector mediated gene transfer
The introduction of foreign DNA into eukaryotic cells has become a standard and indispensable technique in molecular biology, vaccinology and therapy. Our lab has gained a long experience, more than 20 years, on viral genome manipulation and employment as viral vectors. We are mainly focused on Bovine Herpesvirus type 4 (BoHV-4)- based vector, which was created in our lab by cloning an non-pathogenic strain of BoHV-4 genome as a bacterial artificial chromosome (BAC) which we extensively use as an antigen delivery platform for recombinant vaccines construction an oncolysis. Further, 3th generation replicating uncompetent lentiviral vectors are used for gene knocked-down by siRNA/miRNA and CRISPER cas9 approach.
- Study of in vivo and in vitro experimental models for the evaluation of osteogenesis and osteointegration, cellular and tissue interaction with new generation bioactive surfaces and nanomaterials, cell therapy approaches for osteochondrogenesis;
- Histological and anatomical study for the setting up and validation of new animal models in the pre-clinical research lung field.
The applied techniques are morphometric analysis , morphostructural histology and dynamic histomorphometry, SEM and TEM ultrastructural analysis, cytologic assays, cyto- and histo-chemistry, immunofluorescence.